Double negative T cell-NK cell axis orchestrates antitumor immunity against triple negative breast cancer

Abstract Triple-negative breast cancer (TNBC) is hard to treat because of the development chemoresistance and metastasis. It recently found that combination chemotherapy immune checkpoint blockade can significantly improve TNBC treatment. However, low response rate immunotherapy greatly hampers application TNBC. urgent find new paradigms for We previously transferring thymocytes into Ja281 KO mice could inhibit E0771 formation. Herein, we further determine underlying mechanism. splenocytes, CD4 +, or CD8 +T cells not induce antitumor immunity. Transferring thymic double-negative T (DN T) tumor Treating cell-transferred with anti-TCRβ anti-TCRγδ antibodies two days before inoculation, grew in treated but anti-TCRγδ-treated mice. These results indicated cell transfer-induced immunity was mediated by TCRαβ +DN cells. iNKT MAIT DN did affect cell-transfer-induced Only NK depletion abrogated transfer-mediated using an antibody deplete macrophage, suggesting are essential T-mediated against E0771. Antibody circulating transferred immunity, mediating tissue-resident Collectively, these data indicate T/NK axis orchestrates Supported Washington State University College Pharmacy Pharmaceutical Sciences Start-up funds